siLNA constructs are very efficient in mediating sequence specific gene silencing (see Western Blot below). LNA nucleotides in the 3’-ends induce strong biostability and incorporation of 1-5 isolated LNA nucleotides in the sense strand lead to further biostability and significantly reduced off-target effects.
|100 nM siRNA siLNA sisiLNA|
|mock 186 187 189 188 188 188 sictr|
In the reporter assay in Cos7 cells expressing GFP fused to 5’UTR of CVB-3, LNA-modified siRNA (siLNA-188), as well as sisiLNA-188 have significantly better silencing activity than unmodified siRNA-188 at concentration 100 nM (Dr. Jens Kurreck and Mariola Dutkiewicz, Freie Universität Berlin, Research in progress).
Self-hybridisation is strong. Due to the strong LNA:LNA base pairing it is important to design LNA-modified oligonucleotides with a minimum of self-complementary segments.
For more information see http://nar.oxfordjournals.org/cgi/reprint/gkm548v1 and http://mct.aacrjournals.org/cgi/content/abstract/6/3/833
Contact RiboTask for further information and help on siLNA design.